Light microscopy immuno-detection : the anti-vicilin antibodies are visualised with Alexa 488 green fluorescence and counterstained with the red fluorescent propidium iodide. (A) Embryo cells 16 DAP showed green fluorescent vicilin bodies (vib) and the initiation of vacuole fragmentation. (B) Note an increase in the number of vicilin bodies and the presence of a multiple vacuolar compartment (*) at 20 DAP. vb, Vascular Bundle; vc, vacuolar compartment. The scale bar corresponds to 4 μm.