Table 1 Comparison between structurally similar 2-OG dependent proteins
From: Distribution and prediction of catalytic domains in 2-oxoglutarate dependent dioxygenases
| Â | Taurine dioxygenase (TauD) | Alkylsulfatase (AtsK) |
|---|---|---|
Organism | Escherichia coli | Pseudomonas putida |
alpha/beta content | Â | Â |
DSBH (core) | β (5–8,14-17) | β (1–7;13–16) |
Extended | β (9–10), α (1–6) | β (8–12), α (3–5) |
PDB id | 1GQW (Ref.[229]) | 1OIK (Ref.[227]) |
Sequence identity (%) | 41-42 | |
Z score (rmsd) | 31.8-34.4 (1.2-1.3) | |
Active site geometry (amino acid nos.) | Â | Â |
Sphere of radius 5 A0 (Fe/2-OG/Substrate) | 18 | 17 |
Identical residues | 13 | |
Reaction catalyzed | sulfate cleaving activity | |
Substrate profile | taurine (sulfonic acids) | aliphatic sulfate esters |
| Â | Phytanoyl -CoA hydroxylase(PAHX) | Pentalenolactone hydrolase(PtlH) |
Organism | Homo sapiens | Streptomyces avermitilis |
alpha/beta content | Â | Â |
DSBH (core) | β (6,8-13) β (2-4, 7-10) |  |
Extended | β (1–2,5,15) β (1), a (1-6) |  |
PDB id | 2A1X (Ref.[220]) | 2RDN (Ref.[226]) |
Sequence identity (%) | 20 | |
Z score (rmsd) | 17.1 (2.9) | |
Amino acids within sphere of radius 5 A 0 (Fe/2-OG/Substrate) | 12 | 18 |
Reaction catalyzed | hydroxylation | ring closure |
Substrate profile | phytanoyl CoA (medium chain) | 1-deoxypentalenic acid |