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Table 1 Amplification and sequencing success rates of the five candidate loci for 36 Dendrobium species

From: The loci recommended as universal barcodes for plants on the basis of floristic studies may not work with congeneric species as exemplified by DNA barcoding of Dendrobium species

Locus No. of samples used for PCR Amplification No. of amplicons obtained Amplification success No. of finished sequences generated Sequencing success
ITS 292 289 98.97% 269 93.08%
matK 292 290 99.32% 267 92.07%
rbcL 194 188 96.91% 174 92.55%
rpoB 249 247 99.2% 232 93.93%
rpoC1 249 249 100% 238 95.58%