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Table 1 Amplification and sequencing success rates of the five candidate loci for 36 Dendrobium species

From: The loci recommended as universal barcodes for plants on the basis of floristic studies may not work with congeneric species as exemplified by DNA barcoding of Dendrobium species

Locus

No. of samples used for PCR Amplification

No. of amplicons obtained

Amplification success

No. of finished sequences generated

Sequencing success

ITS

292

289

98.97%

269

93.08%

matK

292

290

99.32%

267

92.07%

rbcL

194

188

96.91%

174

92.55%

rpoB

249

247

99.2%

232

93.93%

rpoC1

249

249

100%

238

95.58%