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Table 2 Primer sequences and nucleotide dispensation order used in pyrosequencing assays

From: Copy number variations of the ATP-binding cassette transporter ABCC6 gene and its pseudogenes

Assay # Genes Template Primer Sequence Dispensation order Amplicon size (bp)
      123456789  
1 ABCC6-ABCC6P1 gDNA Forward 5’-TGAGGGAGCCAGGCTAGA-3’   129
    Reverse 5’-Biotin-GAGGGGAAGGGAGAGATTAGC-3’   
    Sequencing 5’-GCCTGGCCCTGCCGC-3’ GTAGC TGC T  
2 ABCC6-ABCC6P1-ABCC6P2 gDNA Forward 5’-Biotin-TCCCATCTACCTCCTCTTCATC-3’   76
    Reverse 5’-ATCTTGGCTTTGAAGAGTGG-3’   
    Sequencing 5’-TGGCTTTGAAGAGTGG-3’ CGACAT CT  
3 ABCC6-ABCC6P1-ABCC6P2 cDNA Forward 5’-Biotin-CGGGGCAGGGGGTCTGGAAC-3’   195
    Reverse 5’-ATCTTGGCTTTGAAGAGTGG-3’   
    Sequencing 5’-TGGCTTTGAAGAGTGG-3’ CGACAT CT  
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