Skip to main content

Table 1 Characteristics of the designed primers specific for Rosaceae PAs biosynthesis genes used in this study

From: Designing universal primers for the isolation of DNA sequences encoding Proanthocyanidins biosynthetic enzymes in Crataegus aronia

Gene name

Primer

Primer sequence (5' → 3')

Tm°Ca

ANR

ANRFwd1

CCCACCTMACAGCACTACAA b

53.8

ANRRev1

TACCGACCAGAAGCAGATTC

51.8

ANRFwd2

GGAGTGATTTGGAGTTCTTG

49.7

ANRRev2

GGAAAATCTCCAAACTCAGT

47.7

ANS

ANSFwd1

TTTGAYCTTCCCATTGAGCA

49.7

ANSRev1

GCATTTTGGGTAGTAGTTGA

47.7

ANSFwd2

CAGCTTGAGTGGGAGGAYTA

53.8

ANSRev2

GCAGGCCRGGAACCATGTTG

57.9

4-CL

4-CLFwd1

CCTGGAGAGATTTGCATCAG

51.8

4-CLRev1

AATACTCGATTTATTCTTTTATA

42.8

4-CLFwd2

ATCATGAAAGGTTAYCTTAATG

45.5

4-CLRev2

GGAATGGCTTCGATGAAAAA

47.7

CHI

CHIFwd1

AGGGGGTYGGAGATTCAGGG

55.9

CHIRev1

GGGAAGKTTTGATCYTTGAA

45.6

CHIFwd2

TTCGTGAAGTTCACGGCGAT

51.8

CHIRev2

TGGGAAGGTCTGATCTTTGA

49.7

C4H

C4HFwd1

CTCCGYATGGGRCAGCGCAA

60

C4HRev1

TTGTTGTACATCATMAGCTG

47.7

C4HFwd2

CAGCTBATGATGTACAACAA

47.7

C4HRev2

TGGATTTCRGGGTGGTTCAC

53.8

CHS

CHSFwd1

AAGGCCATYAAGGAATGGGG

53.8

CHSRev1

AATGTRAGCCCDACTTCACG

53.8

CHSFwd2

AAGGAATGGGGHCAGCCCAA

55.9

CHSRev2

CTCACYTCRTCCAAAATAAA

47.7

DFR

DFRFwd1

CCTGACGCTGTGGAAGGCGG

60

DFRRev1

TAATGGTRATGAAATCAATG

43.6

DFRFwd2

TGCASCGGAGTGTTYCATGT

53.8

DFRRev2

TGAGGCTTGGTGGCATRGATGG

58.6

LAR

LARFwd1

GTTCGTMGCYGAAGCCAGCC

60

LARRev1

TGGAACYGATCCAACGGTGG

55.9

LARFwd2

AGGGCYRATCCGGTKGAACC

60

LARRev2

AGGGTSCGRCCAATTTTCTT

51.8

F3H

F3HFwd1

GACATGTCCGGYGGCAAAAAGGG

60.6

F3HRev1

CTCATCTTCTTCTTGTACAT

45.6

F3HFwd2

ACGTGGATCACCGTTCAACC

53.8

F3HRev2

GGTTGAACGGTGATCCACGT

53.8

PAL

PALFwd1

ATCGATGTTTCRAGGAACAA

47.7

PALRev1

AAAGAGTTAACATCTTGGTT

43.6

PALRev2

AAAAATGTRGAAGACATGAG

45.6

  1. a Melting temperature.
  2. b Degenerate nucleotides codes where B (C/G/T), D (A/G/T), H (A/C/T), K (G/T), M (A/C), N (A/C/G/T), R (A/G), S (G/C), V (A/C/G), W (A/T), Y (C/T).