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Table 2 Species tested for specificity of amplification primer set and detection probe

From: Direct detection of nasal Staphylococcus aureus carriage via helicase-dependent isothermal amplification and chip hybridization

Organism Source of isolate Characteristics Molecular assay
S. schleiferi ATCC # 43808 Coagulase-positive negative
S. capitis ATCC # 35661 Coagulase-negative negative
S. epidermidis ATCC # 12228 Coagulase-negative negative
S. haemolyticus ATCC # 29970 Coagulase-negative negative
S. hominis ATCC # 700236 Coagulase-negative negative
S. lugdunensis ATCC # 43809 Coagulase-negative negative
S. saprophyticus ATCC # 15305 Coagulase-negative negative
S. succinus ATCC # 700337 Coagulase-negative negative
Bacillus subtilis ATCC # 23859 gram-positive negative
Clostridium difficile ATCC # BAA-1382 gram-positive negative
Enterococcus faecalis ATCC # 700802 gram-positive negative
Enterococcus faecium ATCC # 51559 gram-positive negative
Micrococcus luteus ATCC # 10240 gram-positive negative
Mycobacterium abscessus ATCC # 19977 gram-positive negative
Streptococcus agalactiae ATCC # 13813 gram-positive negative
Streptococcus pneumoniae ATCC # 6308 gram-positive negative
Acinetobacter baumannii ATCC # 17978 gram-negative negative
Citrobacter freundii ATCC # 8090 gram-negative negative
Escherichia coli ATCC # 4157 gram-negative negative
Klebsiella pneumoniae ATCC # 13883 gram-negative negative
Neisseria gonorrhoeae ATCC # 53420 gram-negative negative
Pseudomonas putida ATCC # 47054 gram-negative negative
Candida albicans ATCC # 18804   negative
Saccharomyces cerevisiae Strain S288C   negative
Homo sapiens Roche Cat. #11691112001 negative
  1. Isolated microbial colonies were suspended in TN-Buffer (see Materials and Methods section), lysed by incubation with achromopeptidase and/or boiling, and the equivalent of in the order of 106 cells (or 20 ng purified yeast DNA or 80 ng human DNA) was added to each HDA molecular assay reaction.