Figure 1

Neurons visualized with SYTO 13 or Hoechst exhibit different outcomes during time-lapse microscopy. (A) Neurons visualized with Hoescht (blue) remained viable over 6 h. Neurons stained with SYTO 13 (green) became PI-positive by 6 h, indicative of necrotic cell death. White arrows indicate cells that became PI-positive in each frame. (B) Repeated measures quantitation of nuclear area demonstrated no changes regardless of PI uptake.