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Figure 1 | BMC Research Notes

Figure 1

From: Compatibility of SYTO 13 and Hoechst 33342 for longitudinal imaging of neuron viability and cell death

Figure 1

Neurons visualized with SYTO 13 or Hoechst exhibit different outcomes during time-lapse microscopy. (A) Neurons visualized with Hoescht (blue) remained viable over 6 h. Neurons stained with SYTO 13 (green) became PI-positive by 6 h, indicative of necrotic cell death. White arrows indicate cells that became PI-positive in each frame. (B) Repeated measures quantitation of nuclear area demonstrated no changes regardless of PI uptake.

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