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Table 2 RNA purification from tomato and Arabidopsis leaves, petunia flowers and Botrytis cinerea mycelium by LogSpin protocol and by commercial kits

From: LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues

   

RNA yield1

Absorbance

 

Organism

Protocol

μg/100 mg

260/280

260/230

1

Tomato

TRI2

31.3 ± 6.2

2.07 ± 0.01

2.27 ± 0.01

3

Tomato

RNeasy3

26.7 ± 13.3

2.11 ± 0.00

2.33 ± 0.04

2

Tomato

LogSpin

25.1 ± 1.8

2.14 ± 0.01

2.30 ± 0.01

4

Arabidopsis

TRI1

21.1 ± 5.9

2.13 ± 0.00

2.05 ± 0.12

5

Arabidopsis

RNeasy3

16.3 ± 3.8

2.10 ± 0.01

2.28 ± 0.01

6

Arabidopsis

LogSpin

15.5 ± 3.6

2.15 ± 0.01

2.29 ± 0.01

7

Petunia

TRI2

37.8 ± 5.5

1.90 ± 0.06

1.30 ± 0.25

8

Petunia

RNeasy3

14.7 ± 1.0

2.07 ± 0.02

2.02 ± 0.14

9

Petunia

LogSpin

13.6 ± 0.5

2.06 ± 0.02

2.21 ± 0.01

10

B. cinerea

KIT4

31.0 ± 3.4

2.12 ± 0.00

2.14 ± 0.00

11

B. cinerea

LogSpin

83.3 ± 18.9

1.87 ± 0.07

2.05 ± 0.08

  1. 1RNA yield ± SE (n = 3-10).
  2. 2Tri-reagent, Sigma-Aldrich.
  3. 3RNeasy, Qiagen.
  4. 4Norgen.