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Table 2 RNA purification from tomato and Arabidopsis leaves, petunia flowers and Botrytis cinerea mycelium by LogSpin protocol and by commercial kits

From: LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues

    RNA yield1 Absorbance
  Organism Protocol μg/100 mg 260/280 260/230
1 Tomato TRI2 31.3 ± 6.2 2.07 ± 0.01 2.27 ± 0.01
3 Tomato RNeasy3 26.7 ± 13.3 2.11 ± 0.00 2.33 ± 0.04
2 Tomato LogSpin 25.1 ± 1.8 2.14 ± 0.01 2.30 ± 0.01
4 Arabidopsis TRI1 21.1 ± 5.9 2.13 ± 0.00 2.05 ± 0.12
5 Arabidopsis RNeasy3 16.3 ± 3.8 2.10 ± 0.01 2.28 ± 0.01
6 Arabidopsis LogSpin 15.5 ± 3.6 2.15 ± 0.01 2.29 ± 0.01
7 Petunia TRI2 37.8 ± 5.5 1.90 ± 0.06 1.30 ± 0.25
8 Petunia RNeasy3 14.7 ± 1.0 2.07 ± 0.02 2.02 ± 0.14
9 Petunia LogSpin 13.6 ± 0.5 2.06 ± 0.02 2.21 ± 0.01
10 B. cinerea KIT4 31.0 ± 3.4 2.12 ± 0.00 2.14 ± 0.00
11 B. cinerea LogSpin 83.3 ± 18.9 1.87 ± 0.07 2.05 ± 0.08
  1. 1RNA yield ± SE (n = 3-10).
  2. 2Tri-reagent, Sigma-Aldrich.
  3. 3RNeasy, Qiagen.
  4. 4Norgen.
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BMC Research Notes

ISSN: 1756-0500

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