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Table 3 RNA purification by LogSpin, Tri-reagent (TRI) and Qiagen RNeasy kit (KIT) from uninfected and infected Arabidopsis tissue

From: LogSpin: a simple, economical and fast method for RNA isolation from infected or healthy plants and other eukaryotic tissues

   RNA yield1 μg/100 mg Absorbance
  Extracted   260/280 260/230
1 Uninfected - LogSpin 15.5 ± 3.6 2.15 ± 0.01 2.29 ± 0.01
2 Infected - LogSpin 15.8 ± 4.2 2.16 ± 0.01 2.30 ± 0.02
3 Uninfected - KIT 16.3 ± 3.8* 2.10 ± 0.01 2.28 ± 0.01
4 Infected - KIT 9.3 ± 1.7* 2.13 ± 0.01 2.22 ± 0.05
5 Uninfected - TRI 21.1 ± 3.4& 2.13 ± 0.00 2.05 ± 0.07
6 Infected - TRI 10.3 ± 0.81& 2.12 ± 0.04 2.02 ± 0.05
  1. 1RNA yield ± SE, (n = 3-6).
  2. *Statistically significant, t-test, P = 0.007.
  3. &Statistically significant, t-test, P = 0.036.
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BMC Research Notes

ISSN: 1756-0500

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