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Table 1 Mutational analysis of the p53 detection by SSCP and DNA sequencing, p53 nuclear reactivity and p53 status

From: Specific-mutational patterns of p53 gene in bladder transitional cell carcinoma among a group of Iraqi patients exposed to war environmental hazards

Patients No Exon/Codon Codon change Base change Mutation type Amino acid change p53 nuclear reactivity p53 status
1 - - - - - - -
2 - - -   - - -
3 5/184 GAT→AAT G→A Ts Asp184Asn ++ +
4 - - -   - - -
5 7/244 GGC→GGA C→A Tv Gly244Gly - -
6 7/230 Del A - Fr - - +
7 7/NS - -   - ++ +
8 - - -   - - -
9 - - -   - ++ +
10 - - -   - ++ +
11 - - -   - - -
12* 5/154 GGC→GGA C→A Tv Gly154Gly + +
  6/192 CAG→CAC G→C Tv Glu192His   
13 7/244 + A - Fr - - +
14 - - - - - - -
15 7/244 + A - Fr - - +
16 - - - - - ++ +
17 - - - - - + +
18 - - - - - + +
19 - - - - - ++ +
20* 6/196 CGA→CAA G→A Ts Arg196Glu ++ +
  8/283 CGC→CCC G→C Tv Arg283Pro   
21 5/175 CGC→CAC G→A Ts Arg175His ++ +
22 5/176 TGC→GGC T→G Tv Cys176Gly ++ +
23 - - - - - ++ +
24 - - - - - - -
25* 8/293 Del G - Fr - - +
  8/294 GAG→GAA G→A Ts Glu294Glu   
26 6/196 CGA→CAA G→A Ts Arg196Glu ++ +
27 - - - - - ++ +
28 -      ++ +
29 - - -   - ++ +
  1. p53 status; + has alteration (mutation) or immunoreactivty +,++ (IHC), -; no alteration (mutation (−) and IHC (−,+/−), Ts; Transition, Tv; Transvertion, *; Tumor with double mutation, NS; DNA not sequenced but p53 mutation detected by SSCP analysis, - G; deletion of one base (G) at codon 293, - A; deletion of one base (A) at codon 230, + A; Insertion of one base (A) after codon 244/exon 7, Fr; Frameshift.