Specific-mutational patterns of p53 gene in bladder transitional cell carcinoma among a group of Iraqi patients exposed to war environmental hazards

Table 1 Mutational analysis of the p53 detection by SSCP and DNA sequencing, p53 nuclear reactivity and p53 status

Patients No	Exon/Codon	Codon change	Base change	Mutation type	Amino acid change	p53 nuclear reactivity	p53 status
1	-	-	-	-	-	-	-
2	-	-	-		-	-	-
3	5/184	GAT→AAT	G→A	Ts	Asp184Asn	++	+
4	-	-	-		-	-	-
5	7/244	GGC→GGA	C→A	Tv	Gly244Gly	-	-
6	7/230	Del A	-	Fr	-	-	+
7	7/NS	-	-		-	++	+
8	-	-	-		-	-	-
9	-	-	-		-	++	+
10	-	-	-		-	++	+
11	-	-	-		-	-	-
12*	5/154	GGC→GGA	C→A	Tv	Gly154Gly	+	+
	6/192	CAG→CAC	G→C	Tv	Glu192His
13	7/244	+ A	-	Fr	-	-	+
14	-	-	-	-	-	-	-
15	7/244	+ A	-	Fr	-	-	+
16	-	-	-	-	-	++	+
17	-	-	-	-	-	+	+
18	-	-	-	-	-	+	+
19	-	-	-	-	-	++	+
20*	6/196	CGA→CAA	G→A	Ts	Arg196Glu	++	+
	8/283	CGC→CCC	G→C	Tv	Arg283Pro
21	5/175	CGC→CAC	G→A	Ts	Arg175His	++	+
22	5/176	TGC→GGC	T→G	Tv	Cys176Gly	++	+
23	-	-	-	-	-	++	+
24	-	-	-	-	-	-	-
25*	8/293	Del G	-	Fr	-	-	+
	8/294	GAG→GAA	G→A	Ts	Glu294Glu
26	6/196	CGA→CAA	G→A	Ts	Arg196Glu	++	+
27	-	-	-	-	-	++	+
28	-					++	+
29	-	-	-		-	++	+

p53 status; + has alteration (mutation) or immunoreactivty +,++ (IHC), -; no alteration (mutation (−) and IHC (−,+/−), Ts; Transition, Tv; Transvertion, *; Tumor with double mutation, NS; DNA not sequenced but p53 mutation detected by SSCP analysis, - G; deletion of one base (G) at codon 293, - A; deletion of one base (A) at codon 230, + A; Insertion of one base (A) after codon 244/exon 7, Fr; Frameshift.

ISSN: 1756-0500