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Figure 2 | BMC Research Notes

Figure 2

From: Altered specificity of single-chain antibody fragments bound to pandemic H1N1-2009 influenza virus after conversion of the phage-bound to the soluble form

Figure 2

Reactivity of soluble scFv No. 29 against H1N1pdm-, sH1N1-, and mock-infected MDCK cells in IFA. IFA against H1N1pdm-, sH1N1-, and mock-infected MDCK cells was performed to examine the ability of soluble scFv No. 29 to bind virus. Following fixation of the cells using 3.6% formaldehyde and 0.4% Triton-X, 1.25 μg of scFv No. 29 was added and detected with an anti-myc tag MoAb and FITC-conjugated anti-mouse IgG.

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