Figure 3From: Analysis of T-DNA alleles of flavonoid biosynthesis genes in Arabidopsis ecotype ColumbiaPCR confirmation of homozygous insertion lines for described tt alleles. T-DNA insertions were confirmed using T-DNA and gene-specific primers, while intact genes were confirmed using two gene-specific primers spanning the mapped T-DNA insertion site. Homozygous lines are indicated by the presence of a T-DNA insertion but not an intact gene.Back to article page