Figure 2

Map of the integration vector pLL102. The spectinomycin resistance gene spc(pGB2) and replication functions repA(pGB2) are both derived from pGB2 [24] and are functional in E. coli. The tetracycline resistance gene tet(pT181) is expressed in S. aureus. The attP 2 site allows for site-specific recombination between attP 2 and an engineered attB 2 site in the S. aureus chromosome. The plasmid is maintained in S. aureus in a single-copy, integrated form. The plasmid sequence has been deposited to GenBank under the accession number JN639000.