Map of the integration vector pLL102. The spectinomycin resistance gene spc(pGB2) and replication functions repA(pGB2) are both derived from pGB2  and are functional in E. coli. The tetracycline resistance gene tet(pT181) is expressed in S. aureus. The attP 2 site allows for site-specific recombination between attP 2 and an engineered attB 2 site in the S. aureus chromosome. The plasmid is maintained in S. aureus in a single-copy, integrated form. The plasmid sequence has been deposited to GenBank under the accession number JN639000.