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Table 2 Bacterial strains and plasmids used in this study

From: A single copy integration vector that integrates at an engineered site on the Staphylococcus aureus chromosome

Strain or Plasmid Relevant Characteristics Reference or Source
Staphylococcus aureus   
RN4220 Restriction deficient laboratory strain [26]
CYL12337 RN4220 attB 1 This study
CYL12348 RN4220 attB 2 This study
CYL12349 CYL12348 (pYL112Δ19) This study
CYL12376 CYL12349 (pLL102) This study
E. coli   
DH5α Strain for cloning and plasmid maintenance Invitrogen
XL1-Blue Strain for cloning and plasmid maintenance Stratagene
pCL52.2 Temperature-sensitive cloning vector [27]
pLL3961 Derivative of pCL52.2 carrying attP 1 and the L54a int gene This study
pCL25 Carries wild-type attP of L54a [2]
pKOR1 Used for allele replacement in S. aureus [21]
pYL112Δ19 Encodes integrase of phage L54a [3]
pLL102 Derivative of pCL25 carrying attP 2 This study