Novel approach for deriving genome wide SNP analysis data from archived blood spots

BMC Research Notes

Table 1 Concentrations and A260/A280 for 20 samples after WGA (before amplification, concentrations too low for detection)

Sample	Allele size(s) after extraction and microsatellite PCR	Concentration (ng/μl) after WGA	A260:A280 after WGA	Allele size(s) after WGA and microsatellite PCR	Do direct extraction and WGA microsatellite results match?
1	119	612	2.10	119	✓
2	182	561	2.06	182	✓
3	119, 142	555	1.88	119, 142	✓
4	120	609	2.14	121	✓
5	119, 139	587	1.84	119, 139	✓
6	119, 149	580	1.80	119, 149	✓
7	120, 140	521	1.98	120, 140	✓
8	119, 137	534	1.97	119, 137	✓
9	121, 147	575	1.62	No peaks	X – WGA failure
10	118, 144	600	1.85	118, 144	✓
11	119	451	2.01	120	✓
12	119, 148	603	2.03	119, 148	✓
13	117	601	2.14	118	✓
14	120, 140	742	1.98	120, 141	✓
15	119, 142	598	1.86	120, 142	✓
16	118, 140	561	1.87	118, 140	✓
17	119, 137	604	2.05	119, 137	✓
18	121, 144	624	2.14	144	X – Allele drop out
19	142	612	2.07	141	✓
20	119, 146	709	1.95	119, 146	✓

ISSN: 1756-0500