The bench scientist's guide to statistical analysis of RNA-Seq data

BMC Research Notes

Table 1 Post sequencing analysis of raw reads

Sample	Treatment	Flowcell lane	Number of reads	Q.S. (median)	Q.S. (interquartile range)
1	Ambient	4	36,408,402	34	26-36
2	Elevated O₃	4	28,554,551	34	26-36
3	Ambient	5	16,862,414	34	29-37
4	Elevated O₃	5	17,575,844	34	29-37
5	Ambient	6	31,889,531	34	28-37
6	Elevated O₃	6	37,605,167	34	28-37

For each sample, the total number of reads and read quality score (QS) is listed. A QS of 34 indicates one sequencing error per 4,000 base pairs. Generally, a QS over 20 (1% error rate) is considered acceptable for RNA-Seq. One control (−) and one elevated ozone (+) replicate were pooled and run on a single lane of the flow-cell.

ISSN: 1756-0500