Example western blots from three starting samples. In the top panel; (A) myoblast sample (20 μg protein was added to each lane) fractionated into nuclei, cytoplasmic and mitochondrial samples. Each fraction was run side-by-side on the same blot and then probed separately against each of the three primary antibodies, Histone-H3, GAPDH and CoxIV, to validate fraction purity. This was repeated for; (B) myotubes (20 μg protein per lane) and (C) AT tissue (15 μg protein per lane). In the bottom panel; marker protein fraction expression was measured as OD per resultant band area and was expressed in arbitrary units. The histogram data are representative of the mean (± SEM) of five separate experiments.