Figure 3
From: A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue
Comparison of fractions generated from a muscle source by a kit versus the described method. Example western blot to compare sample purity generated after using a commercial cell fractionation kit (K = kit) versus the method reported here (NK = not kit). The kit was used to generate nuclear and cytoplasmic fractions only, these were compared for purity and yield against the method described by probing both fractions for (A) GAPDH and (B) Histone-H3. The C2C12 myotube fractions obtained from both methods were analysed on the same western blot (20 μg protein was loaded from each fraction).