Table 2 Purity analysis of the yield of sub-compartmental marker proteins
From: A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue
Fraction/Marker | Myoblasts | Myotubes | AT Tissue |
|---|---|---|---|
Nuclei/Histone H3 (%) | 92.7 ± 1.3 | 86.4 ± 1.4 | 88.0 ± 0.9 |
Cyto/GAPDH (%) | 92.4 ± 0.5 | 93.9 ± 1.4 | 87.8 ± 0.3 |
Mito/CoxIV (%) | 85.6 ± 0.9 | 92.1 ± 0.5 | 91.2 ± 0.1 |