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Table 2 Purity analysis of the yield of sub-compartmental marker proteins

From: A simple protocol for the subcellular fractionation of skeletal muscle cells and tissue

Fraction/Marker Myoblasts Myotubes AT Tissue
Nuclei/Histone H3 (%) 92.7 ± 1.3 86.4 ± 1.4 88.0 ± 0.9
Cyto/GAPDH (%) 92.4 ± 0.5 93.9 ± 1.4 87.8 ± 0.3
Mito/CoxIV (%) 85.6 ± 0.9 92.1 ± 0.5 91.2 ± 0.1
  1. The percentage shown in the table is obtained by the ratio between OD values of Histone H3, GAPDH and CoxIV (all n = 5, mean ± SEM) in an individual target compartment divided by the sum of the OD values present in all compartments in each sample.