Figure 3

Dose–response of HGF effects on apoptosis induction by CDDP in vitro. The effect of HGF on SK-OV-3 ovarian cancer cells is analysed by flow cytometry. SK-OV-3 cells are pretreated for 48 h with HGF at the reported concentrations and then exposed to 20 μM CDDP for 48 h. A representative experiment out of five performed is shown. The Y-axis shows the percentage of live cells, i.e. cells that did not display either early or late apoptotic features. These cells did not express phosphatidylserine on their surface (Annexin V-FITC binding negative), did not display mitochondrial depolarisation (they were not stained with tetramethylrhodamine methyl ester, TMRM) and were not permeable to propidium iodide (PI), as determined using multiparametric fluorescence activated cell sorter analyses.