Immunohistochemistry | |||||||
---|---|---|---|---|---|---|---|
Antibody | Epitope | Source/ Clonality | Epitope retrieval | Blocking | Dilution | Incubation | Reaction system used for detection |
GdC15 (SCBT) | C-terminal | goat/polyclonal | Proteinase K (30Â min, RT) | UV-Block (45Â min) | 1:1000 (UV Block) | 30Â min (RT) | Vectastain elite kit (goat IgG) |
GdQ13 (SCBT) | C-terminal | goat/polyclonal | citrate buffer (pH 6, 5 min, pressure cooker) | UV-Block (45 min) | 1: 300 (UV Block) | o.n. (4°C) | Vectastain elite kit (goat IgG) |
GdA (Jeschke et al. 2006) | Mixed glycan/protein epitope | mouse/ monoclonal | citrate buffer (pH 6, 5 min, pressure cooker) | 1.5% horse serum (20 min) | 1:3000 (DAKO dil.) | o.n. (4°C) | Vectastain elite kit (mouse IgG) |
Competitive enzyme immuno assay (EIA) | |||||||
Antibody | Epitope | Source/ Clonality | Competing peptide | Blocking | Dilution | Incubation | Reaction system used for detection |
GdQ13 (SCBT) | C-terminal | goat/ polyclonal | GdQ13-P (SCBT) | 1% BSA, 0.5% NaN3 in PBS | 1.5Â ng/ml | 1Â hour (RT) | POD-strepavidin |
GdN20 (SCBT) | N-terminal | goat/ polyclonal | GdN20-P (SCBT) | 1% BSA, 0.5% NaN3 in PBS | 6Â ng/ml | 1Â hour (RT) | POD-strepavidin |