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Table 1 Characterization of ten microsatellite loci for brown mussel Perna perna

From: Characterization of ten highly polymorphic microsatellite loci for the intertidal mussel Perna perna, and cross species amplification within the genus

Locus name (Genebank no.) Primer sequences Repeat motif Clone size (bp) PCR annealing (°C) A Size range (bp) HE HO FIS Cross amplification (positive/total)
Perper-01 F-TGGAACTTAGGGCCTTCCTC (AC)13 176 56 9 158-183 0.8075 0.45 0.46311** 3/5
(JX183697) R-TCCAATTCTGTGAAATCATTGAC
Perper-02 F-CCCGGTTTTAAGTGGTAGATG (GT)15 113 57 6 110-137 0.3112 0.3000 0.06173 0/5
(JX183698) R-AGCAAACGAAGGACAAATCG
Perper-05 F-TCAGTGCCGCGTGATAATAC (TG)15 127 59 16 111-150 0.885 1.000 −0.10465 4/5
(JX183699) R-TCCAATTACGTTTGTTTTTGC  
Perper-08 F-AATGTTCAATGTCGACAGACTATG (ACAG)7 129 56 5 110-126 0.7604 0.5789 0.26394* 0/5
(JX183700) R-TTCTGAAGCACTGGATGTGG  
Perper-11 F-TTAACGTTGAAATCCGTGAGG (GT)9…(GT)13 133 58 16 70-167 0.9044 0.4667 0.51000** 4/5
(JX183701) R-CATTCCAATCCCACGCATAC  
Perper-16 F-TGTGATTTAAAGTTGGACTTGTTTC (AC)26 134 56 8 104-130 0.5606 0.2353 0.60000** 0/5
(JX183702) R-TGATTGGATCAAAATTAAACGTG  
Perper-20 F-ATGTCAATGTGCACAACACG (ACAG)15 245 55 27 202-382 0.9525 0.9000 0.08065 0/5
(JX183703) R-CGTGTATTGGCGACTTTTTATC  
Perper-26 F-CACCACCCTTACAAAGACGTG (AC)11 102 53 11 92-113 0.845 0.7500 0.13767 5/5
(JX183704) R-TTTCACTTGGCGATTAGTATGC  
Perper-27 F-CCCAATTTAACGGGAACAAC (AC)27 167 56 13 103-193 0.8449 0.9474 −0.09459 4/5
(JX183705) R-TTATCATCACCACTTTAAGTTACCC  
Perper-29 F-TTCCATTTCTAGACATCTCTGTCG (AC)11 80 56 5 64-78 0.6763 0.95 −0.38314 5/5
(JX183706) R-TCAGGTGACAGCAGCCTGAC
  1. Locus name and GeneBank accession number, primer sequence, motif repetition, clone size, PCR annealing temperature, number of alleles per locus, fragment size range, gene diversity, observed heterozygosity and inbreeding coefficient (* indicates significant values at p < 0.05 and ** at p < 0.001, after multiple tests correction). Cross amplification on P. canaliculus with five individuals.