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Table 1 Putative glycosyl hydrolases obtained from subclones expressing cellulase activities from the cow rumen metagenome

From: Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene

BAC clone Subclone First blastx match Expect Identity/Similarity(%)
13-I03 p21 β-glucosidase (B. formatexigens DSM 14469, ZP_05346034) 2.00 × 10-23 37/51
13-N07 p296 Cel8B (F. succinogenes, ABU45499) 8.00 × 10-176 89/94
14-B22 p13 CH5 (Uncultured microorganism, AFO64636) 0 78/86
14-F03 p75 Cel9B (F. succinogenes, AAC44386) 0 71/83
3-M02 p28 Cel9B (F. succinogenes, AAC44386) 0 63/75
3-N18 p20 β-glucosidase (P. bergensis DSM 17361, ZP_06005092) 0 70/79
3-N18 p13 β-glucosidase (Bacteroides sp. 2_1_7, ZP_05286991) 2.00 × 10-115 50/66
3-P24 p44 Cel9B (F. succinogenes, AAC44386) 0 71/83
4-E05 p13 β-glucosidase (P. bergensis DSM 17361, ZP_06005092) 4 × 10-29 60/72
4-E05 p10 β-xylosidase (uncultured rumen bacterium,CAP07659) 1 × 10-117 60/71
5-C07 p44c Cel5H (F. succinogenes, ABU45500) 7.00 × 10-60 39/56
5-I05 p20 β -glucosidase related glycosidases (Ruminococcus obeum A2-162, CBL22609) 9.00 × 10-13 31/50
5-K18 p52 β-glucosidase (B. formatexigens DSM 14469, ZP_05346034) 2.00 × 10-36 43/56
6-C02 p2 Cel5H (F. succinogenes, ABU45500) 0 53/69
6-I10 p35 Cel5H (F. succinogenes, ABU45500) 0 53/69
6-K13 p41 Cel5H (F. succinogenes, ABU45500) 7.00 × 10-60 39/56
6-L14 p61 Cel5H (F. succinogenes, ABU45500) 1.00 × 10-5 40/60
7-A06 p24 putative xylanase (Bacteroides fragilis 3_1_12, ZP_05281078) 2.00 × 10-9 29/45
7-D10 p19 β-glucosidase (P. bergensis DSM 17361, ZP_06005092) 0 65/75
7-L24 p1 β-glucosidase (P. bergensis DSM 17361, ZP_06005092) 4.00 × 10-116 62/73