Table 1 Putative glycosyl hydrolases obtained from subclones expressing cellulase activities from the cow rumen metagenome
BAC clone | Subclone | First blastx match | Expect | Identity/Similarity(%) |
|---|---|---|---|---|
13-I03 | p21 | β-glucosidase (B. formatexigens DSM 14469, ZP_05346034) | 2.00 × 10-23 | 37/51 |
13-N07 | p296 | Cel8B (F. succinogenes, ABU45499) | 8.00 × 10-176 | 89/94 |
14-B22 | p13 | CH5 (Uncultured microorganism, AFO64636) | 0 | 78/86 |
14-F03 | p75 | Cel9B (F. succinogenes, AAC44386) | 0 | 71/83 |
3-M02 | p28 | Cel9B (F. succinogenes, AAC44386) | 0 | 63/75 |
3-N18 | p20 | β-glucosidase (P. bergensis DSM 17361, ZP_06005092) | 0 | 70/79 |
3-N18 | p13 | β-glucosidase (Bacteroides sp. 2_1_7, ZP_05286991) | 2.00 × 10-115 | 50/66 |
3-P24 | p44 | Cel9B (F. succinogenes, AAC44386) | 0 | 71/83 |
4-E05 | p13 | β-glucosidase (P. bergensis DSM 17361, ZP_06005092) | 4 × 10-29 | 60/72 |
4-E05 | p10 | β-xylosidase (uncultured rumen bacterium,CAP07659) | 1 × 10-117 | 60/71 |
5-C07 | p44c | Cel5H (F. succinogenes, ABU45500) | 7.00 × 10-60 | 39/56 |
5-I05 | p20 | β -glucosidase related glycosidases (Ruminococcus obeum A2-162, CBL22609) | 9.00 × 10-13 | 31/50 |
5-K18 | p52 | β-glucosidase (B. formatexigens DSM 14469, ZP_05346034) | 2.00 × 10-36 | 43/56 |
6-C02 | p2 | Cel5H (F. succinogenes, ABU45500) | 0 | 53/69 |
6-I10 | p35 | Cel5H (F. succinogenes, ABU45500) | 0 | 53/69 |
6-K13 | p41 | Cel5H (F. succinogenes, ABU45500) | 7.00 × 10-60 | 39/56 |
6-L14 | p61 | Cel5H (F. succinogenes, ABU45500) | 1.00 × 10-5 | 40/60 |
7-A06 | p24 | putative xylanase (Bacteroides fragilis 3_1_12, ZP_05281078) | 2.00 × 10-9 | 29/45 |
7-D10 | p19 | β-glucosidase (P. bergensis DSM 17361, ZP_06005092) | 0 | 65/75 |
7-L24 | p1 | β-glucosidase (P. bergensis DSM 17361, ZP_06005092) | 4.00 × 10-116 | 62/73 |