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Table 3 Substrate specificity of the protein crude extracts from cultures of subclones expressing cellulase activity

From: Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene

Substrate Relative specific activity (%)
  p44 p75 p13 p2 p35 p44c p61 p296 p41 p28
Barley glucan ((1–3,1-4)-β-D-glucan) 100 5.9 15.6 9.2 5.1 20.3 11.1 6.5 2.4 5.3
Lichenan ((1–3,1-4)-β-D-glucan) 80.4 5.6 23.0 5.1 100 28.4 6.0 23.9 1.1 3.3
Carboxymethylcellulose (1,4-β-D-glucan) 53.5 1.2 100 100 50.5 73.0 42.2 78.9 16.8 7.5
Methyl cellulose (1,4-β-D-glucan) 5.3 0.4 12.5 14.2 6.5 8.7 5.8 14.1 0.5 0.3
Avicel (1,4-β-D-glucan) 18.0 0.8 44.8 72.6 21.7 21.1 6.9 62.9 0.7 1.2
Xylan from birchwood (1,4-β-D-xylan) 5.3 0.3 21.3 16.8 6.2 4.9 1.2 18.1 0.2 0.2
Xylan from oat spelt (1,4-β-D-xylan) 12.3 0.6 27.7 18.8 11.4 10.4 5.2 29.8 0.6 1.4
Oat gum ((1–3,1-4)-β-D-glucan) 5.9 100 71.0 14.9 45.5 100 100 100 100 100
Filter paper 4.1 0.2 11.1 7.9 4.6 3.4 1.0 11.8 0.1 0.2