Table 3 Substrate specificity of the protein crude extracts from cultures of subclones expressing cellulase activity
Substrate | Relative specific activity (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Â | p44 | p75 | p13 | p2 | p35 | p44c | p61 | p296 | p41 | p28 |
Barley glucan ((1–3,1-4)-β-D-glucan) | 100 | 5.9 | 15.6 | 9.2 | 5.1 | 20.3 | 11.1 | 6.5 | 2.4 | 5.3 |
Lichenan ((1–3,1-4)-β-D-glucan) | 80.4 | 5.6 | 23.0 | 5.1 | 100 | 28.4 | 6.0 | 23.9 | 1.1 | 3.3 |
Carboxymethylcellulose (1,4-β-D-glucan) | 53.5 | 1.2 | 100 | 100 | 50.5 | 73.0 | 42.2 | 78.9 | 16.8 | 7.5 |
Methyl cellulose (1,4-β-D-glucan) | 5.3 | 0.4 | 12.5 | 14.2 | 6.5 | 8.7 | 5.8 | 14.1 | 0.5 | 0.3 |
Avicel (1,4-β-D-glucan) | 18.0 | 0.8 | 44.8 | 72.6 | 21.7 | 21.1 | 6.9 | 62.9 | 0.7 | 1.2 |
Xylan from birchwood (1,4-β-D-xylan) | 5.3 | 0.3 | 21.3 | 16.8 | 6.2 | 4.9 | 1.2 | 18.1 | 0.2 | 0.2 |
Xylan from oat spelt (1,4-β-D-xylan) | 12.3 | 0.6 | 27.7 | 18.8 | 11.4 | 10.4 | 5.2 | 29.8 | 0.6 | 1.4 |
Oat gum ((1–3,1-4)-β-D-glucan) | 5.9 | 100 | 71.0 | 14.9 | 45.5 | 100 | 100 | 100 | 100 | 100 |
Filter paper | 4.1 | 0.2 | 11.1 | 7.9 | 4.6 | 3.4 | 1.0 | 11.8 | 0.1 | 0.2 |