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Table 4 Substrate specificity of Cel14b22

From: Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene

Substrates Specific activitya(U/mg) ± SD Relative activity (%)
Oat gum ((1–3,1-4)-β-D-glucan) 368.22 ± 2.77 100
Barley β-glucan ((1–3,1-4)-β-D-glucan) 172.94 ± 9.92 47
Carboxymethyl cellulose (1,4-β-D-glucan) 110.42 ± 4.07 30
Lichenan ((1–3,1-4)-β-D-glucan) 86.48 ± 4.45 23
Oat spelt xylan (1,4-β-D-xylan) 39.79 ± 3.62 11
Methyl cellulose (1,4-β-D-glucan) 39.41 ± 3.72 11
Birchwood xylan (1,4-β-D-xylan) 38.29 ± 5.44 10
Avicel (1,4-β-D-glucan) 21.96 ± 3.40 6
Filter paper 20.78 ± 6.97 6
  1. a The incubation time for activity measurement was 15 min, performed in triplicate and the standard deviations were calculated.