Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene

Table 5 Effects of metal ions, chelating agent, and detergent on the enzyme activity of Cel14b22

Reagent	Concentration	Relative activity (%) ± standard deviation
Control	-	100
CoCl₂	10 mM	107.9 ± 4.7
CaCl₂	10 mM	102.2 ± 5.4
CrCl₂	10 mM	68.4 ± 1.6
CuSO₄	10 mM	21.4 ± 0.6
ZnCl₂	10 mM	61.5 ± 0.7
MgCl₂	10 mM	85.0 ± 4.6
FeCl₃	10 mM	12.8 ± 2.3
MnCl₂	10 mM	155.2 ± 5.4
KCl	10 mM	103.7 ± 6.0
NaCl	10 mM	105.5 ± 4.4
EDTA	1 mM	84.0 ± 4.0
SDS	1% w/v	0

ISSN: 1756-0500