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Table 5 Effects of metal ions, chelating agent, and detergent on the enzyme activity of Cel14b22

From: Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene

Reagent Concentration Relative activity (%) ± standard deviation
Control - 100
CoCl2 10 mM 107.9 ± 4.7
CaCl2 10 mM 102.2 ± 5.4
CrCl2 10 mM 68.4 ± 1.6
CuSO4 10 mM 21.4 ± 0.6
ZnCl2 10 mM 61.5 ± 0.7
MgCl2 10 mM 85.0 ± 4.6
FeCl3 10 mM 12.8 ± 2.3
MnCl2 10 mM 155.2 ± 5.4
KCl 10 mM 103.7 ± 6.0
NaCl 10 mM 105.5 ± 4.4
EDTA 1 mM 84.0 ± 4.0
SDS 1% w/v 0