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Table 1 DNA constructs created to simulate DICER1 hot-spot mutations

From: DICER1 RNase IIIb domain mutations are infrequent in testicular germ cell tumours

Variant* Oligonucleotide sequences^
1705_1709  
Reference sequence 5-GGTGCTTGGTTATGAGGTAGTCCaaaatcgcatctcccaggaattctaagCGCTGGTAACAATCTGAGGG-3
3-CCACGAACCAATACTCCATCAGGttttagcgtagagggtccttaagattgGCGACCATTGTTAGACTCCC-5
c.5113G>A p.E1705K 5-GGTGCTTGGTTATGAGGTAGTCCaaaatcgcatctcccaggaattT taagCGCTGGTAACAATCTGAGGG-3
3-CCACGAACCAATACTCCATCAGGttttagcgtagagggtccttaaA attgGCGACCATTGTTAGACTCCC-5
c.5125G>A p.D1709N 5-GGTGCTTGGTTATGAGGTAGTCCaaaatcgcatT tcccaggaattctaagCGCTGGTAACAATCTGAGGG-3
3-CCACGAACCAATACTCCATCAGGttttagcgtaA agggtccttaagattgGCGACCATTGTTAGACTCCC-5
c.5126A>G p.D1709G 5-GGTGCTTGGTTATGAGGTAGTCCaaaatcgcaC ctcccaggaattctaagCGCTGGTAACAATCTGAGGG-3
3-CCACGAACCAATACTCCATCAGGttttagcgtG gagggtccttaagattgGCGACCATTGTTAGACTCCC-5
c.5127T>A p.D1709E 5-GGTGCTTGGTTATGAGGTAGTCCaaaatcgcT tctcccaggaattctaagCGCTGGTAACAATCTGAGGG-3
3-CCACGAACCAATACTCCATCAGGttttagcgA agagggtccttaagattgGCGACCATTGTTAGACTCCC-5
1810_1813  
Reference sequence 5-CATGTAAATGGCACCAGCAAgcgactcaaaaatatcccccatggCCTTTGGAACTTCAATATCCTCTT-3
3-GTACATTTACCGTGGTCGTTcgctgagtttttatagggggtaccGGAAACCTTGAAGTTATAGGAGAA-5
c.5428G>T p.D1810Y 5-CATGTAAATGGCACCAGCAAgcgactcaaaaatatA ccccatggCCTTTGGAACTTCAATATCCTCTT-3
3-GTACATTTACCGTGGTCGTTcgctgagtttttataT ggggtaccGGAAACCTTGAAGTTATAGGAGAA-5
c.5437G>C p.E1813Q 5-CATGTAAATGGCACCAGCAAgcgactG aaaaatatcccccatggCCTTTGGAACTTCAATATCCTCTT-3
3-GTACATTTACCGTGGTCGTTcgctgaC tttttatagggggtaccGGAAACCTTGAAGTTATAGGAGAA-5
  1. * Nucleotide and amino acid numbering are based on DICER1 reference sequence [GenBank:NM_177438].
  2. ^ Priming sequences for PCR amplification (using primers listed in Table2) are in capital letters. The nucleotides representing the mutations are in bold.