Table 1 DNA constructs created to simulate DICER1 hot-spot mutations
From: DICER1 RNase IIIb domain mutations are infrequent in testicular germ cell tumours
Variant* | Oligonucleotide sequences^ |
|---|---|
1705_1709 | |
Reference sequence | 5′-GGTGCTTGGTTATGAGGTAGTCCaaaatcgcatctcccaggaattctaagCGCTGGTAACAATCTGAGGG-3′ |
3′-CCACGAACCAATACTCCATCAGGttttagcgtagagggtccttaagattgGCGACCATTGTTAGACTCCC-5′ | |
c.5113G>A p.E1705K | 5′-GGTGCTTGGTTATGAGGTAGTCCaaaatcgcatctcccaggaattT taagCGCTGGTAACAATCTGAGGG-3′ |
3′-CCACGAACCAATACTCCATCAGGttttagcgtagagggtccttaaA attgGCGACCATTGTTAGACTCCC-5′ | |
c.5125G>A p.D1709N | 5′-GGTGCTTGGTTATGAGGTAGTCCaaaatcgcatT tcccaggaattctaagCGCTGGTAACAATCTGAGGG-3′ |
3′-CCACGAACCAATACTCCATCAGGttttagcgtaA agggtccttaagattgGCGACCATTGTTAGACTCCC-5′ | |
c.5126A>G p.D1709G | 5′-GGTGCTTGGTTATGAGGTAGTCCaaaatcgcaC ctcccaggaattctaagCGCTGGTAACAATCTGAGGG-3′ |
3′-CCACGAACCAATACTCCATCAGGttttagcgtG gagggtccttaagattgGCGACCATTGTTAGACTCCC-5′ | |
c.5127T>A p.D1709E | 5′-GGTGCTTGGTTATGAGGTAGTCCaaaatcgcT tctcccaggaattctaagCGCTGGTAACAATCTGAGGG-3′ |
3′-CCACGAACCAATACTCCATCAGGttttagcgA agagggtccttaagattgGCGACCATTGTTAGACTCCC-5′ | |
1810_1813 | |
Reference sequence | 5′-CATGTAAATGGCACCAGCAAgcgactcaaaaatatcccccatggCCTTTGGAACTTCAATATCCTCTT-3′ |
3′-GTACATTTACCGTGGTCGTTcgctgagtttttatagggggtaccGGAAACCTTGAAGTTATAGGAGAA-5′ | |
c.5428G>T p.D1810Y | 5′-CATGTAAATGGCACCAGCAAgcgactcaaaaatatA ccccatggCCTTTGGAACTTCAATATCCTCTT-3′ |
3′-GTACATTTACCGTGGTCGTTcgctgagtttttataT ggggtaccGGAAACCTTGAAGTTATAGGAGAA-5′ | |
c.5437G>C p.E1813Q | 5′-CATGTAAATGGCACCAGCAAgcgactG aaaaatatcccccatggCCTTTGGAACTTCAATATCCTCTT-3′ |
3′-GTACATTTACCGTGGTCGTTcgctgaC tttttatagggggtaccGGAAACCTTGAAGTTATAGGAGAA-5′ |