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Table 2 PCR amplification primers used in this study

From: DICER1 RNase IIIb domain mutations are infrequent in testicular germ cell tumours

Genomic region covered*

Forward primer (5′-3′)

Reverse primer (5′-3′)

Product size

Comment^

chr14:95560431-95560500

GGTGCTTGGTTATGAGGTAGTCC

CCCTCAGATTGTTACCAGCG

70 bp

Product includes codons 1705–1709 of DICER1; primer sequences from this study

chr14:95557604-95557671

CATGTAAATGGCACCAGCAA

AAGAGGATATTGAAGTTCCAAAGG

68 bp

Product includes codons 1810–1813 of DICER1; primer sequences from this study

chr14:95560345-95560533

CTTCTGCACAAGCTTACGGTTCCA

CAGCGATGCAAAGATGGTGTTGT

188 bp

Product includes codons 1705–1709 of DICER1; primer sequences from[10].

chr14:95557565-95557759

TGGACTGCCTGTAAAAGTGG

ACACACCTGCCAGACTGTCTCC

194 bp

Product includes codons 1810–1813 of DICER1; primer sequences from[10].

  1. * Genomic location is based on reference sequence hg19.
  2. ^ Amino acid numbering is based on DICER1 reference sequence [GenBank:NM_177438].