Figure 2
From: High-throughput real-time PCR-based genotyping without DNA purification
Blind Validation Study. A. An allelic discrimination plot depicting the genotyping results for 96 samples genotyped in duplicate. The wild type allele used the VIC probe (y-axis) while the minor allele used the FAM probe (x-axis). Figure 2B. Standard deviation of wild type clusters for the VIC signal. The variance between sample clusters was assessed by calculating the standard deviation of the wild type samples for all of the assays.