Figure 1

Inbreeding strategies for generating G3 mice carrying homozygous ENU-induced mutations . (Left) The G0 male is mutagenized; the G0’ female is a wild type C57BL/6J animal. G1 males are mated to wild type C57BL/6J females, and the resulting G2 daughters are backcrossed to their G1 father to yield G3 mice. (Right) The G0 male is mutagenized; the G0 female (the daughter of another mutagenized male and a wild type C57BL/6J female) also carries ENU-induced mutations. G1 siblings are intercrossed to obtain G2 mice. G3 mice are obtained from intercrosses of G2 siblings.