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Table 1 Oligonucleotide primers used in this study

From: Expression analysis of calmodulin and calmodulin-like genes from rice, Oryza sativa L.

Primer name

Sequence

Positiona

Amplicon size (bp)b

Annealing temp. (°C)

OsCam1-1-F

5- ACCGTGCATTGCCGTATTAG -3

499-518

177

58.3

OsCam1-1-R

5- GCAAGCCTTAACAGATTCAC -3

656-675

OsCML1-F

5- CCAGAAGTGCGTGATCCTGT -3

543-562

184

58.3

OsCML1-R

5- ACTACGGACTACGGCTGTGA -3

707-726

OsCML3-F

5- ACTACAACGAGTTCCTCAAG -3

410-429

180

57.3

OsCML3-R

5- CATCAGAACAGTTGCAAACC -3

570-589

OsCML4-F

5- GCAGGTGAACTACGATGAAT -3

402-421

193

56.3

OsCML4-R

5- TACCCATAGCTGAAGTCCAA -3

575-594

OsCML5-F

5- ATGATGCTCTCCGACCAATA -3

481-500

180

57.3

OsCML5-R

5- CCAAGGCCAAATTAAATGAC -3

641-660

OsCML7-F

5- CCGCATCGTCGCCAAATAAT -3

429-448

193

57.3

OsCML7-R

5- GTCCAAATCACACCGGAATG -3

602-621

OsCML8-F

5- AGATGATGAAGAGGATAGGA -3

539-555

185

56.3

OsCML8-R

5- AAACATAAGGCGGTATGGTA -3

701-720

OsCML9-F

5- TACAAGGAGTTCGTCAAGGT -3

430-449

170

58.3

OsCML9-R

5- GATTCGCTTGAATCATATCGC -3

579-600

OsCML11-F

5- CAACATCTTCTCCTGAGAAT -3

621-640

183

56.3

OsCML11-R

5- ATTCACAAGAGCTCGATCAC -3

784-803

OsCML13-F

5- ATCGAAATGGTGATGGTGAG -3

437-456

193

58.3

OsCML13-R

5- GCATGGTTGTTCTTGTTCAG -3

610-629

OsEF1α- F

5- ATGGTTGTGGAGACCTTC -3

1192-1209

127

58.3

OsEF1α- R

5- TCACCTTGGCACCGGTTG -3

1301-1318

  1. aPosition of the primers from the GenBank sequence given in the materials and methods, where position 1 is the predicted open reading frame start codon and numbered 5 to 3 on the sense strand.
  2. bExpected amplicon size based upon the primer positions on the GenBank sequence.