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Table 2 Estimates of A-to-I RNA editing frequency between Dα6IR2 and Dα6285F sequencing primers

From: Antisense sequencing improves the accuracy and precision of A-to-I editing measurements using the peak height ratio method

  

Editing Site

Sequencing Primer

Expected Editing Rate

398

400

415

416

Dα6IR2

0.95

0.94 ± 0.02

0.96 ± 0.01

0.92 ± 0.03

0.92 ± 0.03

 

0.90

0.88 ± 0.01*

0.90 ± 0.01

0.87 ± 0.00*

0.88 ± 0.01

 

0.75

0.76 ± 0.01

0.74 ± 0.01

0.75 ± 0.02

0.76 ± 0.01

 

0.50

0.51 ± 0.01*

0.50 ± 0.03

0.50 ± 0.02

0.52 ± 0.01*

 

0.25

0.25 ± 0.03

0.24 ± 0.02

0.23 ± 0.01

0.25 ± 0.02

 

0.10

0.11 ± 0.03

0.09 ± 0.01

0.09 ± 0.02

0.09 ± 0.03

 

0.05

0.07 ± 0.03

0.07 ± 0.02

0.08 ± 0.05

0.07 ± 0.05

Dα6285F

0.95

0.85 ± 0.12

0.88 ± 0.17

0.97 ± 0.03

0.97 ± 0.02

 

0.90

0.86 ± 0.05

0.86 ± 0.05

0.95 ± 0.01*

0.93 ± 0.00*

 

0.75

0.66 ± 0.04*

0.79 ± 0.04

0.84 ± 0.03*

0.81 ± 0.03*

 

0.50

0.38 ± 0.02*

0.58 ± 0.02*

0.67 ± 0.01*

0.60 ± 0.05*

 

0.25

0.19 ± 0.03*

0.33 ± 0.04*

0.43 ± 0.04*

0.39 ± 0.13

 

0.10

0.06 ± 0.03

0.17 ± 0.02*

0.33 ± 0.06*

0.23 ± 0.10*

 

0.05

0.04 ± 0.02

0.13 ± 0.04

0.25 ± 0.04*

0.23 ± 0.06*

  1. Values represent the mean ± standard deviation. Values with an asterisk indicate estimated editing frequency is different from the expected editing rate (one-sample t-test vs. expected mean, p < 0.05)