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Table 2 Estimates of A-to-I RNA editing frequency between Dα6IR2 and Dα6285F sequencing primers

From: Antisense sequencing improves the accuracy and precision of A-to-I editing measurements using the peak height ratio method

   Editing Site
Sequencing Primer Expected Editing Rate 398 400 415 416
Dα6IR2 0.95 0.94 ± 0.02 0.96 ± 0.01 0.92 ± 0.03 0.92 ± 0.03
  0.90 0.88 ± 0.01* 0.90 ± 0.01 0.87 ± 0.00* 0.88 ± 0.01
  0.75 0.76 ± 0.01 0.74 ± 0.01 0.75 ± 0.02 0.76 ± 0.01
  0.50 0.51 ± 0.01* 0.50 ± 0.03 0.50 ± 0.02 0.52 ± 0.01*
  0.25 0.25 ± 0.03 0.24 ± 0.02 0.23 ± 0.01 0.25 ± 0.02
  0.10 0.11 ± 0.03 0.09 ± 0.01 0.09 ± 0.02 0.09 ± 0.03
  0.05 0.07 ± 0.03 0.07 ± 0.02 0.08 ± 0.05 0.07 ± 0.05
Dα6285F 0.95 0.85 ± 0.12 0.88 ± 0.17 0.97 ± 0.03 0.97 ± 0.02
  0.90 0.86 ± 0.05 0.86 ± 0.05 0.95 ± 0.01* 0.93 ± 0.00*
  0.75 0.66 ± 0.04* 0.79 ± 0.04 0.84 ± 0.03* 0.81 ± 0.03*
  0.50 0.38 ± 0.02* 0.58 ± 0.02* 0.67 ± 0.01* 0.60 ± 0.05*
  0.25 0.19 ± 0.03* 0.33 ± 0.04* 0.43 ± 0.04* 0.39 ± 0.13
  0.10 0.06 ± 0.03 0.17 ± 0.02* 0.33 ± 0.06* 0.23 ± 0.10*
  0.05 0.04 ± 0.02 0.13 ± 0.04 0.25 ± 0.04* 0.23 ± 0.06*
  1. Values represent the mean ± standard deviation. Values with an asterisk indicate estimated editing frequency is different from the expected editing rate (one-sample t-test vs. expected mean, p < 0.05)