Table 4 Comparison of methods used to measure A-to-I RNA editing

Peak Height Ratio

6 (RNA isolation, RT, PCR, PCR purification, sequencing, analysis)

3

$11.87

$35.62

1

Several

Poisoned Primer Extension

7 (RNA isolation, RT, primer labeling, PCR, gel, imaging, analysis)

1-2

$120.63 F^b$123.40 ³²P

$133.24 F^b$157.84 ³²P

15.0 F^b

17.7 ³²P

One

Restriction Digest

9 (RNA isolation, RT, PCR, purification, RE digestion, purification, gel, imaging, analysis)

2

$10.37 F^b

$123.40 ³²P

$31.10 F^b

$135.70 ³²P

3.50 F^b

15.2 ³²P

One

Ultra High Throughput Sequencing

9 (RNA isolation, RT, PCR(×2), purification(×2), hybridization, sequencing, analysis)

21-28

$1609.80

$4829.39

136

One to Several^c

Clone Counting

10 (RNA isolation, RT, PCR, PCR purification, cloning, transformation, colony screening, colony growth, plasmid purification, sequencing, analysis)

6

$24.48

$561.25

15.8

Several