Table 1 In silico analysis of primer specificity of the primers used in this study
Target | Probe | Sequence (5′ → 3′) | No. of target strains detecteda | Sensitivity (%)b | No. of non-target strains detecteda | Specificity (%)b | Reference | |
|---|---|---|---|---|---|---|---|---|
G. vaginalis | Gard154-454 | Fw | CTCTTGGAAACGGGTGGTAA | 36 (from 40) | 90 | 1 (from 1032184) | 100 | This study |
Rv | TTGCTCCCAATCAAAAGCGGT | 38 (from 40) | 95 | 94 (from 1032184) | 99,99 | |||
GV1 + 3 | Fw | TTACTGGTGTATCACTGTAAGG | [15] | |||||
Rv | CCGTCACAGGCTGAACAGT | |||||||
Gv1 + 2 | Fw | TCCTGTCTACCAAGGCATCC | [13] | |||||
Rv | CGTGTGATAACCGTCAGGTG | |||||||
A. vaginae | Atop109-329 | Fw | GAGTAACACGTGGGCAACCT | 457 (from 467) | 97,86 | 18390 (from 1031757) | 98,22 | This study |
Rv | CCGTGTCTCAGTCCCAATCT | 449 (from 467) | 96,15 | 1356 (from 1031757) | 99,87 | |||
AtovagRT3 | Fw | GGTGAAGCAGTGGAAACACT | 134 (from 467) | 28,69 | 0 (from 1031757) | 100 | [11] | |
Rv | ATTCGCTTCTGCTCGCGCA | 109 (from 467) | 23,34 | 2 (from 1031757) | 100 | |||
Atop167-587 | Fw | GCGAATATGGGAAAGCTCCG | 117 (from 467) | 25,05 | 0 (from 1031757) | 100 | [10] | |
Rv | TCATGGCCCAGAAGACCGCC | 115 (from 467) | 24,63 | 0 (from 1031757) | 100 | |||
Lactobacillus spp. | AM Lacto | Fw | TGATGCATAGCCGAGTTGAG | 7353 (from 12936) | 56,84 | 2513 (from 1019288) | 99,75 | This study |
Rv | AGCCGAAACCCTTCTTCACT | 5858 (from 12936) | 45,28 | 1027 (from 1019288) | 99,9 | |||
New Lacto | Fw | TGGAAACAGRTGCTAATACCG | 11680 (from 12936) | 90,29 | 7069 (from 1019288) | 99,31 | [16] | |
Rv | GTCCATTGTGGAAGATTCCC | 10113 (from 12936) | 78,18 | 3311 (from 1019288) | 99,68 | |||
S21 + A19 | Fw | TGCCTAATACATGCAAGTCGA | 9754 (from 12936) | 75,4 | 184796 (from 1019288) | 81,87 | [17] | |
Rv | GTTTGGGCCGTGTCTCAGT | 10157 (from 12936) | 78,52 | 72659 (from 1019288) | 92,87 |