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Table 2 In vitro analysis of primer specificity of the primers used in this study

From: In silico vs in vitro analysis of primer specificity for the detection of Gardnerella vaginalis, Atopobium vaginae and Lactobacillus spp.

Target Probe Annealing temp(°C) No. of target strains detecteda Sensitivity (%)b No. of non-target strains detecteda Specificity (%)b Reference
G. vaginalis Gard154-454 60 3 (from 3) 100.0 0 (from 6) 100.0 This study
   62 12 (from 12) 100.0 0 (from 34) 100.0  
  GV1 + 3 60 3(from 3) 100.0 0 (from 6) 100.0 [15]
   62 10 (from 12) 83.3 0 (from 34) 100.0  
  Gv1 + 2 60 3 (from 3) 100.0 0 (from 6) 100.0 [13]
   62 11 (from 12) 91.7 0 (from 34) 100.0  
A. vaginae Atop109-329 60 3 (from 3) 100.0 5 (from 6) 16.7 This study
   62 12 (from 12) 100.0 15 (from 24) 37.5  
   66 12 (from 12) 100.0 3 (from 34) 91.2  
  AtovagRT3 60 12 (from 12) 100.0 1 (from 24) 95.8 [11]
   62 12 (from 12) 100.0 2 (from 34) 94.1  
  Atop167-587 60 2 (from 3) 66.7 0 (from 6) 100.0 [10]
   62 11 (from 12) 91.6 4 (from 34) 88.3  
Lactobacillus spp. AM Lacto 60 2 (from 12) 16.7 1 (from 24) 95.8 This study
   58 2 (from 12) 16.7 3 (from 34) 91.2  
  New Lacto 60 5 (from 12) 41.7 18 (from 34) 47,1 [16]
   62 1 (from 12) 8.3 8 (from 24) 66.7  
  S21 + A19 60 5 (from 12) 41.7 24 (from 24) 0 [17]
   62 6 (from 12) 50.0 34 (from 34) 0  
  1. aCalculated using ProbeMatch (last accession, May 2012) with the following data set options: Strain – Both; Source – Both; Size – > 1200 bp; Quality – Both.
  2. bFormula: Specificity = (nts/Tnts)*100 (nts: number of non-target strains undetected, Tnts: total number of non-target strains tested).
  3. Sensitivity = (ts/Tts)*100 (ts: number of target strains detected, Tts: total number of target strains tested).