Table 2 In vitro analysis of primer specificity of the primers used in this study
Target | Probe | Annealing temp(°C) | No. of target strains detecteda | Sensitivity (%)b | No. of non-target strains detecteda | Specificity (%)b | Reference |
|---|---|---|---|---|---|---|---|
G. vaginalis | Gard154-454 | 60 | 3 (from 3) | 100.0 | 0 (from 6) | 100.0 | This study |
62 | 12 (from 12) | 100.0 | 0 (from 34) | 100.0 | |||
GV1 + 3 | 60 | 3(from 3) | 100.0 | 0 (from 6) | 100.0 | [15] | |
62 | 10 (from 12) | 83.3 | 0 (from 34) | 100.0 | |||
Gv1 + 2 | 60 | 3 (from 3) | 100.0 | 0 (from 6) | 100.0 | [13] | |
62 | 11 (from 12) | 91.7 | 0 (from 34) | 100.0 | |||
A. vaginae | Atop109-329 | 60 | 3 (from 3) | 100.0 | 5 (from 6) | 16.7 | This study |
62 | 12 (from 12) | 100.0 | 15 (from 24) | 37.5 | |||
66 | 12 (from 12) | 100.0 | 3 (from 34) | 91.2 | |||
AtovagRT3 | 60 | 12 (from 12) | 100.0 | 1 (from 24) | 95.8 | [11] | |
62 | 12 (from 12) | 100.0 | 2 (from 34) | 94.1 | |||
Atop167-587 | 60 | 2 (from 3) | 66.7 | 0 (from 6) | 100.0 | [10] | |
62 | 11 (from 12) | 91.6 | 4 (from 34) | 88.3 | |||
Lactobacillus spp. | AM Lacto | 60 | 2 (from 12) | 16.7 | 1 (from 24) | 95.8 | This study |
58 | 2 (from 12) | 16.7 | 3 (from 34) | 91.2 | |||
New Lacto | 60 | 5 (from 12) | 41.7 | 18 (from 34) | 47,1 | [16] | |
62 | 1 (from 12) | 8.3 | 8 (from 24) | 66.7 | |||
S21 + A19 | 60 | 5 (from 12) | 41.7 | 24 (from 24) | 0 | [17] | |
62 | 6 (from 12) | 50.0 | 34 (from 34) | 0 |