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Figure 2 | BMC Research Notes

Figure 2

From: Generation of Trichoderma atroviride mutants with constitutively activated G protein signaling by using geneticin resistance as selection marker

Figure 2

Screening of T. atroviridec transformants for the presence of the nptII gene. Genomic DNA was isolated from geneticin-resistant transformants and screened by PCR for the presence of the nptII gene using primers pII99Fw and pII99Rev (Table1) which yielded a specific amplicon of 888-bp. The ~600-bp band amplified from transformant 1 indicates the presence of an additional incomplete nptII gene copy. M, DNA size standard; -, wild-type; 1–8, transformants; +, plasmid pII99.

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