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Figure 4 | BMC Research Notes

Figure 4

From: Generation of Trichoderma atroviride mutants with constitutively activated G protein signaling by using geneticin resistance as selection marker

Figure 4

Screening of T. atroviride co-transformants for the presence of the nptII gene. Genomic DNA was isolated from 22 geneticin-resistant transformants resulting from co-transformation of T. atroviride P1 with plasmids pKAtga3 and pII99 and analyzed by PCR for integration of the nptII gene using primers pII99Fw and pII99Rev. M, DNA size standard; +, plasmid pII99; 1–22, transformants; -, wild-type.

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BMC Research Notes

ISSN: 1756-0500

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