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Figure 6 | BMC Research Notes

Figure 6

From: Generation of Trichoderma atroviride mutants with constitutively activated G protein signaling by using geneticin resistance as selection marker

Figure 6

Confirmation of integration of a full-length tga3Q207Lgene in ectopic co-transformants 3/3 and 4/5. The presence of a complete copy of the tga3Q207L gene in co-transformants 3/3 (lane 2) and 4/5 (lane 3) was confirmed by PCR using primers tga3PromFw, which binds to the 5′ non-coding region of tga3, and M13Fw, which binds to the backbone of plasmid pKAtga3 (Table1). Both co-transformants yielded the expected 3343-bp amplicon. Lane 1, wild-type; lane 4, plasmid pKAtga3.

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BMC Research Notes

ISSN: 1756-0500

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