Generation of Trichoderma atroviride mutants with constitutively activated G protein signaling by using geneticin resistance as selection marker

Table 1 Oligonucleotides used

name	forward primer (5^′ to 3^′)	reverse primer (5^′ to 3^′)
tga3Phusion	ACGTTGGCGGGCTAAGGAGTGAACG	CAAACATGCTAAAGAAGAACGATC
pII99	ACTCGTCAAGAAGGCGATAGAAGGC	CAAGCAAGGTAAGTGAACGACCCG
tga3Probe	GAATCCAAACCTCCTCGTCC	GCAGAATCCATCAGGTAAAACTC
tga3PromFw	CATCAATCCATCATCAGTCCAAG
tga3TermRev		CCTCTCTCATTTGCTCAACTC
M13Fw	GTTTTCCCAGTCACGAC
tga3LocusCheck	CTTGGAGTCTGCGTGCG
Mut-tga3	GACTACCAAGCAGAATCCGG	TGATTGAACCGCCATAGCAG

ISSN: 1756-0500