Figure 4

Alterations in the origin activity are paralleled by SIDD changes in the ura4 origin region. Relationship between the extent of SIDD and origin activity in the ura4 origin region in (A) wild-type, and, (B-D) ARS deletion S. pombe strains [18]. The map of the concerned region, locations of ARS elements, restriction sites (Av=AvrII, B=BamHI, Bg=BglII, Bs=BstBI, C=ClaI RI=EcoRI, RV=EcoRV, H=HinDIII, N=NruI, Ns=NsiI,S=SmaI) and the 2D autoradiograms of different fragments shown on the top of each section have been reproduced from [18] with permission. The arrows mark the bubble arc signal, indicative of the origin activity of the detected fragment. The lowest G(x) value for each ARS element is shown in kcal/mol near the valley.