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Table 1 Nutrient solution modified from Lullien et al.[47]

From: Morphological and genetic changes induced by excess Zn in roots of Medicago truncatula A17 and a Zn accumulating mutant

  Modified Lullien Solution
Compound 0.5x 0.5x High Zn 0.5x -N 0.5x High Zn -N
K 2 SO 4 0.26 mM 0.26 mM 0.26 mM 0.26 mM
MgSO 4 0.125 mM 0.125 mM 0.125 mM 0.125 mM
Na 2 -EDTA 25 μM 27 μM 25 μM 27 μM
H 3 BO 3 15 μM 15 μM 15 μM 15 μM
MnSO 4 5 μM 5 μM 5 μM 5 μM
ZnSO 4 *7H 2 O 0.35 μM 18 μM 0.35 μM 18 μM
CuSO 4 0.1 μM 0.1 μM 0.1 μM 0.1 μM
Na 2 MoO 4 0.5 μM 0.5 μM 0.5 μM 0.5 μM
CoCl 2 0.02 μM 0.02 μM 0.02 μM 0.02 μM
CaCl 2 0.5 mM 0.5 mM 0.5 mM 0.5 mM
NH 4 NO 3 2.5 mM 2.5 mM -- --
KH 2 PO 4 1.375 mM 1.375 mM 1.375 mM 1.375 mM
K 2 HPO 4 1.375 mM 1.375 mM 1.375 mM 1.375 mM
Fe(SO 4 )*7H 2 0 25 μM 25 μM 25 μM 25 μM
  1. Bold and italicized values indicate changes in concentration necessary for treatments, -- indicates nutrients were absent.
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