Identification and characterization of a salt stress-inducible zinc finger protein from Festuca arundinacea

BMC Research Notes

Table 1 Gene target information.

Name	Top Reference Alignment	Arabidopsis Homolog	Position	Forward primer Reverse primer 5'-3'	% efficiency
eIF1	ref\|XP_478516.1\| translational initiation factor eIF1 Oryza sativa	AT1G20010	97	GAAGAACGTCTCAAATTTCCTCG CAGTTGCTCAGAAACCATGAATC	99
GST24	ref\|XP_463733.1\| glutathione S-transferase GST 24 \| Oryza sativa	AT1G10370	80	AGAAGATCTCACCAACAAGAGC TCGCCGTGGAGGAGAAC	95
Lipoxy-genase L2	ref\|XP_469655.1\| lipoxygenase L-2; Oryza sativa	AT1G72520	361	CACGAGCCTGCCATTGATTA TGTGGTTGTTCTTGACGATGA	98
MAPK1	ref\|XP_470659.1\| Putative MAP kinase 1 Oryza sativa	AT3G45640	148	CCACGGAGAATTTGATAAAGGAAATAC TCCATCAGATTATTCGCTCAAATCAAG	95
FaZnF	TIGR Transcript Assembly TA567_4606 Putative Zn finger Festuca arundinacea	n/a	632	TGTGCTACCTCACCGTCA TCAGGATGCCCAACAACTAGA	96
GAPDH	TIGR Transcript Assembly TA626_4606 Glycerol aldehyde dehydrogenase Festuca arundinacea	n/a	951	ATGGGTTATGTTGAGGAGGATT TTGACGAAGTTGTCGTTCAGAG	99
UBC	TIGR Transcript Assembly DT703874 Ubiquitin conjugating enzyme Festuca arundinacea	n/a	239	CGGCGGCTTCAACTACA CTCGCCAGCATAGAGTG	102

Quantitative PCR primers for each gene and the relative position of the amplicon (center) in the Tall Fescue genes/contigs are indicated. Amplicon length was designed to be between 75-125 bp. Gene target references are also noted along with the PCR efficiency of each primer set. Where applicable, the corresponding Arabidopsis gene reference number of genes found to be up-regulated in Arabidopsis plants overexpressing OsSAP11 [32] are included.

ISSN: 1756-0500