Table 3 Primers used for specificity testing, after selection based on amplification efficiency, quantification limit, and intra-run reproducibility (data not presented)
Species (reference) | Correlation standard curve | Amplification efficiency (%) | Quantification limit (number of bacteria per 25 μl reaction) |
|---|---|---|---|
Aggregatibacter actinomycetemcomitans[35] | 0.99 | 89 | 25 |
Fusobacterium spp. [37] | 0.99 | 94 | 4 |
Parvimonas micros[39] | 0.99 | 91 | 2 |
Porphyromonas gingivalis[33] | 0.99 | 95 | 9 |
Prevotella intermedia[35] | 0.99 | 91 | 11 |
Treponema denticola[33] | 0.99 | 95 | 150 |
Tannerella forsythia[40] | 0.99 | 93 | 25 |
Streptococcus mutans[36] | 0.98 | 115 | 37 |