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Table 4 Intercycler portability of the different assays on the different thermal cyclers, by comparison of the limits of reliable quantification, i.e. the most diluted standard that could be used to calculate the standard curve, expressed here as number of cells present in the most diluted standard reaction mixture

From: Optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogens

Assay

ABI 7300 (2.5/22.5)a

iQ5 (2.5/22.5)

LC 480 (1/9)

LC 480 (2/8)

Species

Reference

Aggregatibacter actinomycetemcomitans

Maeda et al. [35]

26

26

10

2

Fusobacterium spp.

Martin et al. [37]

4

4

2

3

Parvimonas micros

Bartz et al. [39]

2

1

1

2

Porphyromonas gingivalis

Hyvarinen et al. [33]

9

90

36

7

Prevotella intermedia

Maeda et al. [35]

11

11

4

9

Streptococcus mutans

Yoshida et al. [36]

37

37

15

3

Tannerella forsythia

Shelburne et al. [40]

25

25

10

2

Treponema denticola

Hyvarinen et al. [33]

150

15

6

1

  1. Legend
  2. a: Volume of DNA extract (μl)/Volume of total mixture (μl).