DNA extract characterization process for microbial detection methods development and validation

BMC Research Notes

Table 1 Extraction methods by application and processes

Method^a	Intended sample type	Lysis method	Purification method
Reference	General	Mechanical - Bead Beat	Phenol Chloroform
PrecipB	Biofilm	Mechanical - Bead Beat	Precipitation and Silica Column
MagBeads	General	Mechanical - Bead Beat	Magnetic Beads
PrecipS	Soil	Mechanical - Bead Beat	Precipitation and Silica Column
ChemLysis	Gram Negative	Chemical - Enzymatic	Silica Column
PrecipG	General	Mechanical - Bead Beat	Precipitation and Silica Column

^a DNA extraction kits are represented based on the fundamental methods utilized by each kit rather than kit names. Kits included MoBio PowerBiofilm (precipB), MoBio PowerSoil (precipS), MoBio UltraClean (precipG), Qiagen DNAeasy with Gram Negative lysis protocol (chemLysis), and Idaho Technologies Platinum Path (magBeads). Certain commercial equipment, instruments, or materials are identified in this paper in order to specify the experimental procedure adequately. Such identification is not intended to imply recommendations or endorsement by NIST, nor is it intended to imply that the materials or equipment identified are necessarily the best available for the purpose.

ISSN: 1756-0500