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Table 3 DNA primers used in quantity qPCR assays

From: DNA extract characterization process for microbial detection methods development and validation

Organism

Gene target (amplicon size)a

Name

Sequence

Conb

AEc

Ref.

E. coli

lacZ (70)

lacZF1

CCT GAG GCC GAT ACT GTC GT

3

0.99

[52]

  

lacZR1

TTG GTG TAG ATG GGC GCA T

3

  

B. thailandensis

fliC (62)

fliCF1

AGC AGA TCT CGG AAG TGA ACC

2

0.92

This study

  

fliCR1

GAG GAT GTT CTT GCC GTT GT

2

  

B. cereus

pc-plc (144)

PCER-F

GGA TTC ATG GAG CGG CAG TA

3

0.95

[53]

  

PCER-3R

GCT TAC CTG TCA TTG GTG TAA CTT CA

2

  

S. cerevisiae

26Sd (124)

YEAST-F

GAG TCG AGT TGT TTG GGA ATG C

3

0.91

[54]

  

YEST-R

TCT CTT TCC AAA GTT CTT TTC ATC TTT

2

  
  1. a Amplicon size in base pairs.
  2. b Optimized primer concentration (μM).
  3. c Amplification Efficiency, calculated using the slope of the standard curve.
  4. d Assay targeted the D1/D2 variable region of 26S.