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Table 3 DNA primers used in quantity qPCR assays

From: DNA extract characterization process for microbial detection methods development and validation

Organism Gene target (amplicon size)a Name Sequence Conb AEc Ref.
E. coli lacZ (70) lacZF1 CCT GAG GCC GAT ACT GTC GT 3 0.99 [52]
   lacZR1 TTG GTG TAG ATG GGC GCA T 3   
B. thailandensis fliC (62) fliCF1 AGC AGA TCT CGG AAG TGA ACC 2 0.92 This study
   fliCR1 GAG GAT GTT CTT GCC GTT GT 2   
B. cereus pc-plc (144) PCER-F GGA TTC ATG GAG CGG CAG TA 3 0.95 [53]
   PCER-3R GCT TAC CTG TCA TTG GTG TAA CTT CA 2   
S. cerevisiae 26Sd (124) YEAST-F GAG TCG AGT TGT TTG GGA ATG C 3 0.91 [54]
   YEST-R TCT CTT TCC AAA GTT CTT TTC ATC TTT 2   
  1. a Amplicon size in base pairs.
  2. b Optimized primer concentration (μM).
  3. c Amplification Efficiency, calculated using the slope of the standard curve.
  4. d Assay targeted the D1/D2 variable region of 26S.