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Table 2 Most abundantly represented transcripts in a dataset of 7,606 ESTs prepared from a mixed stage unstressed culture of the anhydrobiotic nematode Panagrolaimus superbus.

From: A molecular analysis of desiccation tolerance mechanisms in the anhydrobiotic nematode Panagrolaimus superbus using expressed sequenced tags

Cluster ID

Blast Identity [Organism]

Accession Number* §

BLAST Score & E value

Number of ESTs (Percentage of Total EST Dataset)

PSC00077

Immunodominant antigen (SXP/RAL-2 protein) [Ancylostoma caninum]

ABD98404.1

304

3e-26

79 (1.04)

PSC00006

No significant similarity found

-

-

-

71 (0.93)

PSC00009

No significant similarity found

-

-

-

70 (0.92)

PSC00076

No significant similarity found

-

-

-

54 (0.71)

PSC00137

No significant similarity found

-

-

-

49 (0.64)

PSC00511

Major sperm protein [Caenorhabditis elegans]

NP_494858.1

635

1e-64

40 (0.53)

PSC00155

Hypothetical protein (Y105C5A.8) [C. elegans]

NP_001041003

430

1e-40

39 (0.51)

PSC00051

No significant similarity found

-

-

-

35 (0.46)

PSC01915

Major sperm protein [C. elegans]

NP_494858.1

640

3e-65

30 (0.39)

PSC00043

Yolk protein (vitellogenin) (CEW1-vit-6) [Oscheius sp.]

U35449

280

6e-23

29 (0.38)

PSC00163

No significant similarity found

-

-

-

29

PSC00182

No significant similarity found

-

-

-

29

PSC00025

No significant similarity found

-

-

-

28 (0.37)

PSC00165

Major sperm protein [C. elegans]

NP_494858.1

640

3e-65

27 (0.35)

PSC00883

No significant similarity found

-

-

-

27

PSC00633

No significant similarity found

-

-

-

26 (0.34)

PSC00187

Lysosomal protein (heh-1) [C. elegans]

NP_497671.2

295

3e-25

25 (0.33)

PSC00252

Expressed sequence tag [Meloidogyne chitwoodi]

MCP06382§

358

2e-32

25

PSC00203

No significant similarity found

-

-

-

25

PSC00316

No significant similarity found

-

-

-

25

PSC00610

No significant similarity found

-

-

-

24 (0.31)

PSC00167

Vitellogenin (vit-5) [C. elegans]

NP_508589

238

2e-18

23 (0.30)

PSC00241

No significant similarity found

-

-

-

22 (0.29)

PSC00429

No significant similarity found

-

-

-

22

PSC00179

Major sperm protein [C. elegans]

NP_501781.1

634

1e-64

21 (0.28)

PSC00876

Lipid binding protein (lbp-3) [C. elegans]

NP_001041249

360

9e-33

21

PSC00004

No significant similarity found

-

-

-

21

PSC00010

No significant similarity found

-

-

-

21

PSC00566

Major sperm protein [C. elegans]

NP_494898

633

2e-64

20 (0.26)

PSC00265

60S ribosomal protein L7a [Loa loa]

XP_003139379.1

883

e-93

19 (0.25)

PSC00457

Actin family member [Panagrellus redivivus]

AAM47606.1

1187

3e-148

19

PSC00015

Histone H4d [Xenopus laevis]

NP_001128541.1

469

2e-45

17 (0.22)

PSC00128

Cytochrome c oxidase subunit 1 [Chabertia ovina]

YP_003434131.1

1300

3e-141

17

PSC00326

Major sperm protein [B. malayi]

XP_001902608.1

210

2e-15

17

PSC00122

No significant similarity found

-

-

-

17

PSC00047

60S ribosomal protein L37a [B. malayi]

XP_001902009.1

416

3e-39

16 (0.21)

PSC00764

Expressed sequence tag [M. chitwoodi]

MCP06382_1§

522

4e-51

16

PSC00097

No significant similarity found

-

-

-

16

PSC00962

Expressed sequence tag[Angiostrongylus cantonensis]

AAC00593_1§

198

6e-14

15 (0.19)

PSC00184

Major sperm protein [Dictyocaulus viviparus]

ABW37697.1

624

2e-63

15

PSC00064

Eukaryotic elongation factor 1A [B. xylophilus]

ACZ13348.1

1,348

8e-147

15

PSC00486

Major sperm protein [C. elegans]

NP_494858.1

640

3e-65

15

PSC00127

No significant similarity found

-

-

-

15

PSC00725

No significant similarity found

-

-

-

15

  1. The consensus sequence for each EST was translated using prot4EST [46] and was then subjected to a BLASTP search against a non-redundant custom database comprising NCBI (nr), NemPep + and WormPep sequences
  2. * NCBI (nr) accession numbers are given when available
  3. §Three unigenes had hits only to NemPep EST sequences from the NEMBASE4 database