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Table 5 BLASTX similarity searches of P. superbus unigenes predicted to encode late embryogenesis abundant (LEA) proteins against the NCBI nr and LEAP [http://forge.info.univ-angers.fr/~gh/Leadb/index.php] databases

From: A molecular analysis of desiccation tolerance mechanisms in the anhydrobiotic nematode Panagrolaimus superbus using expressed sequenced tags

Contig ID & [No of ESTs]

Best BLAST hit & [Organism]

Database&Accession Number

BLAST* Bit Score E value

Disordered AAs (%)

GRAVY Index #

PSC00061 [10]

LEA protein [Caenorhabditis briggsae]

NCBI nr

CAP25449

82.8

4e-14

86%

-0.765

PSC00061

LEA protein [Arabidopsis thaliana]

LEAPdb

AAL59922

74.3

8e-16

  

PSC00416 [6]

LEA protein K08H10.1e [Caenorhabditis elegans]

NCBI nr

CCA65580

60.8

1e-7

18%

-0.618

PSC00489

LEA protein [Caenorhabditis elegans]

LEAPdb

AAB69446

80.5

9e-18

  

PSC00489 [2]

LEA3 protein [Glycine max]

NCBI nr

CAA80491.1

68.6

2e-10

10%

-0.810

PSC00416

LEA protein [Caenorhabditis elegans]

LEAPdb

AAB69446

74.7

3e-16

  

PSC00514 [2]

LEA3 protein [Glycine max]

NCBI nr

CAA80491.1

64.7

5e-12

9%

-0.854

PSC00514

LEA protein [Pisum sativum]

LEAPdb

CAF32327

94.0

9e-22

  

PSC00782 [2]

Hypothetical protein [Caenorhabditis briggsae]

NCBI nr

CAP25465

66.2

9e-12

100%

-1.367

PSC00782

LEA-like protein [Arabidopsis thaliana]

LEAPdb

BAB10116

67.4

4e-22

  

PSC01414 [1]

LEA protein [C. briggsae]

NCBI nr

XP_002637990.1

84.7

5e-15

100%

-1.424

PSC01414

LEA-like protein [A. thaliana]

LEAPdb

BAD43695

76.6

1e-16

  

PSC01455 [2]

Hypothetical protein NCU01912 [Neurospora crassa]

NCBI nr

XP_965543.1

62.0

7e-8

99%

-1.034

PSC01455

LEA-like protein [A. thaliana]

LEAPdb

NP_193834

61.2

6e-12

  

PSC01720 [1]

Predicted protein Gls24 [Gemella haemolysans]

NCBI nr

ZP_04776234.1

78.2

8e-13

40%

-0.623

PSC01720

LEA-like protein [A. thaliana]

LEAPdb

BAD43695

90.5

9e-21

  

PSC01853 [1]

Hypothetical protein [Haemophilus influenzae]

NCBI nr

ZP_01786547

67.0

1e-9

100%

-0.988

PSC01853

Hypothetical protein [H. influenzae]

LEAPdb

ZP_01786547

67.0

 

9e-14

 

PSC03871 [2]

Hypothetical protein K08H10.1f [C. elegans]

NCBI nr

CCA65610

42.4

3e-7

77%

-0.656

PSC03871

LEA group 3 protein [Lindernia brevidens]

LEAPdb

ACA49509

54.7

5e-10

  

PSC04142 [1]

Hypothetical protein [Toxoplasma gondii]

NCBI nr

EEE21041

55.8

2e-6

100%

-1.340

PSC04142

LEA-like protein [A. thaliana]

LEAPdb

BAD43695

50.4

4e-09

  

PSC04118 [1]

Protein At5g44310 [A. thaliana]

NCBI nr

AAS49101§

47.0

1e-5

100%

-1.405

PSC04118

LEA-like protein [A. thaliana]

LEAPdB

BAD43695

47.0

2e-9

  

PSC04695 [2]

Hypothetical protein K08H10.1f [C. elegans]

NCBI nr

CCA65610

73.6

1e-11

100%

-1.297

PSC04695

LEA-like protein [A. thaliana]

LEAPdb

BAD43695

67.4

7e-14

  
  1. The higher e-values against the LEAPdb reflect the smaller number of proteins in this database compared to the NCBI nr database
  2. * These BLASTX searches were carried out using the Blosum 62 substitution matrix
  3. ¶% Disordered AAs: AA residues with an IUPRED score above 0.5 can be regarded as disordered [110]
  4. # Grand average of hydropathicity [111]
  5. §The accession numbers BAD43695.1 and AAS49101.1 both correspond to the A. thaliana gene At5g44310