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Table 5 BLASTX similarity searches of P. superbus unigenes predicted to encode late embryogenesis abundant (LEA) proteins against the NCBI nr and LEAP [http://forge.info.univ-angers.fr/~gh/Leadb/index.php] databases

From: A molecular analysis of desiccation tolerance mechanisms in the anhydrobiotic nematode Panagrolaimus superbus using expressed sequenced tags

Contig ID & [No of ESTs] Best BLAST hit & [Organism] Database&Accession Number BLAST* Bit Score E value Disordered AAs (%) GRAVY Index #
PSC00061 [10] LEA protein [Caenorhabditis briggsae] NCBI nr CAP25449 82.8 4e-14 86% -0.765
PSC00061 LEA protein [Arabidopsis thaliana] LEAPdb AAL59922 74.3 8e-16   
PSC00416 [6] LEA protein K08H10.1e [Caenorhabditis elegans] NCBI nr CCA65580 60.8 1e-7 18% -0.618
PSC00489 LEA protein [Caenorhabditis elegans] LEAPdb AAB69446 80.5 9e-18   
PSC00489 [2] LEA3 protein [Glycine max] NCBI nr CAA80491.1 68.6 2e-10 10% -0.810
PSC00416 LEA protein [Caenorhabditis elegans] LEAPdb AAB69446 74.7 3e-16   
PSC00514 [2] LEA3 protein [Glycine max] NCBI nr CAA80491.1 64.7 5e-12 9% -0.854
PSC00514 LEA protein [Pisum sativum] LEAPdb CAF32327 94.0 9e-22   
PSC00782 [2] Hypothetical protein [Caenorhabditis briggsae] NCBI nr CAP25465 66.2 9e-12 100% -1.367
PSC00782 LEA-like protein [Arabidopsis thaliana] LEAPdb BAB10116 67.4 4e-22   
PSC01414 [1] LEA protein [C. briggsae] NCBI nr XP_002637990.1 84.7 5e-15 100% -1.424
PSC01414 LEA-like protein [A. thaliana] LEAPdb BAD43695 76.6 1e-16   
PSC01455 [2] Hypothetical protein NCU01912 [Neurospora crassa] NCBI nr XP_965543.1 62.0 7e-8 99% -1.034
PSC01455 LEA-like protein [A. thaliana] LEAPdb NP_193834 61.2 6e-12   
PSC01720 [1] Predicted protein Gls24 [Gemella haemolysans] NCBI nr ZP_04776234.1 78.2 8e-13 40% -0.623
PSC01720 LEA-like protein [A. thaliana] LEAPdb BAD43695 90.5 9e-21   
PSC01853 [1] Hypothetical protein [Haemophilus influenzae] NCBI nr ZP_01786547 67.0 1e-9 100% -0.988
PSC01853 Hypothetical protein [H. influenzae] LEAPdb ZP_01786547 67.0   9e-14  
PSC03871 [2] Hypothetical protein K08H10.1f [C. elegans] NCBI nr CCA65610 42.4 3e-7 77% -0.656
PSC03871 LEA group 3 protein [Lindernia brevidens] LEAPdb ACA49509 54.7 5e-10   
PSC04142 [1] Hypothetical protein [Toxoplasma gondii] NCBI nr EEE21041 55.8 2e-6 100% -1.340
PSC04142 LEA-like protein [A. thaliana] LEAPdb BAD43695 50.4 4e-09   
PSC04118 [1] Protein At5g44310 [A. thaliana] NCBI nr AAS49101§ 47.0 1e-5 100% -1.405
PSC04118 LEA-like protein [A. thaliana] LEAPdB BAD43695 47.0 2e-9   
PSC04695 [2] Hypothetical protein K08H10.1f [C. elegans] NCBI nr CCA65610 73.6 1e-11 100% -1.297
PSC04695 LEA-like protein [A. thaliana] LEAPdb BAD43695 67.4 7e-14   
  1. The higher e-values against the LEAPdb reflect the smaller number of proteins in this database compared to the NCBI nr database
  2. * These BLASTX searches were carried out using the Blosum 62 substitution matrix
  3. % Disordered AAs: AA residues with an IUPRED score above 0.5 can be regarded as disordered [110]
  4. # Grand average of hydropathicity [111]
  5. §The accession numbers BAD43695.1 and AAS49101.1 both correspond to the A. thaliana gene At5g44310