Figure 4

Processes surrounding neurites are GFAP positive. (A-B) Electron micrographs of cross-sections of explants grown in EN2 after 4 days in culture that have been Immunogold labeled for GFAP. In these images GFAP positive immunolabeling is visible as the electron-rich black dots in the electron micrographs. Axons (arrows) do not retain clear microtubule organization in samples processed for immunolabeling, but were identified on the basis of structure and size. Likely axonal processes are surrounded by GFAP positive glial processes. Scale bar equals 1 μm, m=mitochondria.