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Table 3 All bacteria real-time PCR detection with a universal system in 15 non-diarrheal stool specimens diluted in 1:5 in sterile phosphate buffer, before and after lyophilization (Ct value)

From: Optimized microbial DNA extraction from diarrheic stools

Sample n°

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

Without lyophilization

33.01

22.22

27.99

NA

NA

22.76

42.97

31.76

27.71

27.82

28.18

38.91

35.02

32.25

30.5

With lyophilization

15.61

24.24

21.70

17.41

25.19

16.78

22.02

25.94

20.58

21.47

21.24

27.43

37.34

33.91

24.28

  1. NA, not amplified.